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New scientific highlights- by MUST PIs Majed Chergui and Steve Johnson
The FP-RESOMUS Grant Agreement- now signed by the ETH Zürich and the European Commission
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Nobel Prize in Physics 2018 for groundbreaking inventions: intense ultrafast laser pulses and optical tweezers
Ambizione grant awarded to Elsa Abreu- in Steve Johnson's group
Fabrizio Carbone promoted Associate Professor of Physics- in the EPFL School of Basic Sciences from 1st of August
New scientific highlights- by MUST PIs Ursula Keller, Gebhard Schertler / Jörg Standfuss, Majed Chergui, Peter Hamm
White Paper Photonics Switzerland- presented June 20, 2018 at the Swissmem "Industrietag"

Catching proteins in the act

August 22, 2016

Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography


Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.


Figure. In side view (a) and perpendicular to the membrane (b). The retinal is shown as yellow sticks and the Lys216 side chain covalently binding retinal as blue sticks. Continuous 2FoFc electron density map around retinal and lysine is shown in magenta. Helices A–G are defined based on the header of pdb entry 1QHJ.

Reference: Nogly, P., V. Panneels, G. Nelson, C. Gati, T. Kimura, C. Milne, D. Milathianaki, M. Kubo, W. Wu, C. Conrad, J. Coe, R. Bean, Y. Zhao, P. Båth, R. Dods, R. Harimoorthy, K. R. Beyerlein, J. Rheinberger, D. James, D. DePonte, C. Li, L. Sala, G. J. Williams, M. S. Hunter, J. E. Koglin, P. Berntsen, E. Nango, S. Iwata, H. N. Chapman, P. Fromme, M. Frank, R. Abela, S. Boutet, A. Barty, T. A. White, U. Weierstall, J. Spence, R. Neutze, G. Schertler and J. Standfuss (2016). Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nature Commun. 7: 12314. 10.1038/ncomms12314 Nogly-2016 (1.87 MB).

Also see the the ScienceDaily article


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