Forthcoming Events

11.07.2022 - 15.07.2022, Celeste Hotel, on UCF main campus, Orlando, Florida
05.09.2022 - 09.09.2022, Iseolago hotel, Iseo, Italy.


MUST2022 Conference- succesfully concluded
New scientific highlights- by MUST PIs Chergui and Richardson
FELs of Europe prize for Jeremy Rouxel- “Development or innovative use of advanced instrumentation in the field of FELs”
Ruth Signorell wins Doron prizefor pioneering contributions to the field of fundamental aerosol science
New FAST-Fellow Uwe Thumm at ETH- lectures on Topics in Femto- and Attosecond Science
International Day of Women and Girls in Science- SSPh asked female scientists about their experiences
New scientific highlight- by MUST PIs Milne, Standfuss and Schertler
EU XFEL Young Scientist Award for Camila Bacellar,beamline scientist and group leader of the Alvra endstation at SwissFEL
Prizes for Giulia Mancini and Rebeca Gomez CastilloICO/IUPAP Young Scientist Prize in Optics & Ernst Haber 2021
Nobel Prize in Chemistry awarded to RESOLV Member Benjamin List- for the development of asymmetric organocatalysis
NCCR MUST at Scientifica 2021- Lightning, organic solar cells, and virtual molecules
#NCCRWomen- NCCR MUST celebrates 50 years women’s right to vote in Switzerland
Kick-Off dynaMENT Mentoring for Women in Natural Sciences- with Ursula Keller as plenary speaker

Bill Pedrini, Rafael Abela, Bruce Patterson and co-workers: new paper in Nature Communications

April 3, 2013

Two-dimensional structure from random multiparticle X-ray scattering images using cross-correlations

B. Pedrini, A. Menzel, M. Guizar-Sicairos, V.A. Guzenko, S. Gorelick, C. David, B.D. Patterson & R. Abela
(2013) Nature Communications 4, Article number: 1647, doi:10.1038/ncomms2622

Knowledge of the structure of biological macromolecules, especially in their native environment, is crucial because of the close structure–function relationship. X-ray small-angle scattering is used to determine the shape of particles in solution, but the achievable resolution is limited owing to averaging over particle orientations. In 1977, Kam proposed to obtain additional structural information from the cross-correlation of the scattering intensities.
Here we develop the method in two dimensions, and give a procedure by which the single-particle diffraction pattern is extracted in a model-independent way from the correlations. We demonstrate its application to a large set of synchrotron X-ray scattering images on ensembles of identical, randomly oriented particles of 350 or 200nm in size. The obtained 15nm resolution in the reconstructed shape is independent of the number of scatterers. The
results are discussed in view of proposed ‘snapshot’ scattering by molecules in the liquid phase at X-ray free-electron lasers.


Figure 1 | Overview of the cross-correlation based method applied to the 2D structure determination. The flow of the protocol follows the magenta arrows: (a) the membrane carrying the gold nanostructures is scanned by the X-ray beam, and a number of scattering images, with intensities I, is acquired at different positions. The CCs (C(1), C(2) and C(3)) are calculated as averages over all images, and (b) the single-particle diffraction pattern S is computed. The red bar represents a reciprocal space momentum transfer of 0.1nm1. (c) The 2D electron density r is finally reconstructed using a phasing algorithm. The red bar corresponds to 100 nm. (d) SEM image of a small part of the sample membrane, showing the particles in random orientations. The magnification is an oblique view of a single nanostructure. The dashed orange rectangle covers an area of 146 mm2, which corresponds approximately to the X-ray beam illumination area (FWHM limit of the beam intensity in the two directions). (e) Top SEM view of a single particle, on which the dashed orange contour of the reconstructed shape is superimposed. The red bar again corresponds to 100 nm.

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