Ursula Keller wins “Swiss Nobel” Marcel Benoist Prize
Farewell: the NCCR MUST ended 
MUST2022 Conference
New scientific highlights
FELs of Europe prize for Jeremy Rouxel
Ruth Signorell wins Doron prize
New FAST-Fellow Uwe Thumm at ETH
International Day of Women and Girls in Science
New scientific highlight
EU XFEL Young Scientist Award for Camila Bacellar,
Prizes for Giulia Mancini and Rebeca Gomez Castillo
Nobel Prize in Chemistry awarded to RESOLV Member Benjamin List
Hans Jakob Wörner invited to give the „New Horizons Solvay Lectures” 
Unusual keynote talk at an international scientific conference
NCCR MUST at Scientifica 2021Catching proteins in the act
August 22, 2016Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
Figure. In side view (a) and perpendicular to the membrane (b). The retinal is shown as yellow sticks and the Lys216 side chain covalently binding retinal as blue sticks. Continuous 2Fo−Fc electron density map around retinal and lysine is shown in magenta. Helices A–G are defined based on the header of pdb entry 1QHJ.
Reference: Nogly, P., V. Panneels, G. Nelson, C. Gati, T. Kimura, C. Milne, D. Milathianaki, M. Kubo, W. Wu, C. Conrad, J. Coe, R. Bean, Y. Zhao, P. Båth, R. Dods, R. Harimoorthy, K. R. Beyerlein, J. Rheinberger, D. James, D. DePonte, C. Li, L. Sala, G. J. Williams, M. S. Hunter, J. E. Koglin, P. Berntsen, E. Nango, S. Iwata, H. N. Chapman, P. Fromme, M. Frank, R. Abela, S. Boutet, A. Barty, T. A. White, U. Weierstall, J. Spence, R. Neutze, G. Schertler and J. Standfuss (2016). Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nature Commun. 7: 12314. 10.1038/ncomms12314
Nogly-2016 (1.87 MB).Also see the the ScienceDaily article
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