Forthcoming Events

05.09.2022 - 09.09.2022, Iseolago hotel, Iseo, Italy.

News

MUST2022 Conference- a great success!
New scientific highlights- by MUST PIs Wörner, Chergui, and Richardson
FELs of Europe prize for Jeremy Rouxel- “Development or innovative use of advanced instrumentation in the field of FELs”
Ruth Signorell wins Doron prizefor pioneering contributions to the field of fundamental aerosol science
New FAST-Fellow Uwe Thumm at ETH- lectures on Topics in Femto- and Attosecond Science
International Day of Women and Girls in Science- SSPh asked female scientists about their experiences
New scientific highlight- by MUST PIs Milne, Standfuss and Schertler
EU XFEL Young Scientist Award for Camila Bacellar,beamline scientist and group leader of the Alvra endstation at SwissFEL
Prizes for Giulia Mancini and Rebeca Gomez CastilloICO/IUPAP Young Scientist Prize in Optics & Ernst Haber 2021
Nobel Prize in Chemistry awarded to RESOLV Member Benjamin List- for the development of asymmetric organocatalysis
NCCR MUST at Scientifica 2021- Lightning, organic solar cells, and virtual molecules
#NCCRWomen- NCCR MUST celebrates 50 years women’s right to vote in Switzerland

Non-linear vibrational microscopy

Date Do, 19.05.2011 - Do, 19.05.2011
Time 10.15
Speaker Prof. Dr. Andreas Zumbusch, Department Chemie, Universität Konstanz, 78457 Konstanz
Location Universität Bern, Institut für Angewandte Physik, Gebäude exakte Wissenschaften, Hörsaal B116, Sidlerstrasse 5, 3012 Bern
Program During the last decade, ultrasensitive microscopy has become one of the most important tools in biophysics. Most prominent among the various techniques is confocal fluorescence microscopy. It is a very widespread technique with which sensitivities down to the single molecule detection limit can be achieved. In applications of fluorescence microscopy, however, one faces two problems: i) In most cases, the samples need to be labeled with a fluorophore which can be difficult and can change the properties of the sample. ii) All fluorophores are prone to photobleaching which severely limits the maximum achievable observation times.
In order to solve these problems, recent years have seen the development of a broad variety of different techniques, which generate contrast without the need of external staining. In this talk, Coherent Anti-Stokes Raman Scattering (CARS) microscopy providing chemically selective imaging will be presented 1. CARS microscopy is a non-linear optical technique in which two laser frequencies are used to excite the sample simultaneously. If the frequencies are chosen such that their difference coincides with a vibrational transition of the sample, a strong signal which is higher in energy than the excitation beams is generated. Apart from its biological applications, CARS microscopy has recently attracted a lot of attention as an application field for modern approaches of ultrafast spectroscopy 2,3. As an outlook, I will discuss ways to detect time resolved vibrational spectra of single molecules.
1 M. Müller, A. Zumbusch "Coherent Anti-Stokes Raman Scattering (CARS) microscopy" ChemPhysChem, 8 (2007) 2156- 2169 2 G. Krauss, T. Hanke, A. Sell, D. Träutlein, A. Leitenstorfer. R. Selm. M. Winterhalder, A. Zumbusch "A compact coherent anti-Stokes Raman scattering microscope based on a picosecond two-color Er:fiber laser system", Opt. Lett., 34 (2009) 2847-2849 3 R. Selm, M. Winterhalder, A. Zumbusch, G. Krauß, T. Hanke, A. Sell, A. Leitenstorfer” Ultrabroadband background-free Coherent anti-Stokes Raman Scattering microscopy based on a compact Er:fiber laser system”, Opt. Lett., 35 (2010) 3282- 3284 4 M. Winterhalder, A. Zumbusch, M. Lippitz, M. Orrit, "Towards Far-Field Vibrational Spectroscopy of Single Molecules at Room Temperature" J. Phys. Chem. B, in press
Download (2 KB)
(2 KB)
Link www.iap.unibe.ch
NCCR MUST Office : ETHZ IQE/ULP-HPT H3 | Auguste-Piccard-Hof 1 | 8093 Zurich | E-Mail
The National Centres of Competence in Research (NCCR) are a research instrument of the Swiss National Science Foundation