Forthcoming Events

05.09.2022 - 09.09.2022, Iseolago hotel, Iseo, Italy.

News

MUST2022 Conference- a great success!
New scientific highlights- by MUST PIs Wörner, Chergui, and Richardson
FELs of Europe prize for Jeremy Rouxel- “Development or innovative use of advanced instrumentation in the field of FELs”
Ruth Signorell wins Doron prizefor pioneering contributions to the field of fundamental aerosol science
New FAST-Fellow Uwe Thumm at ETH- lectures on Topics in Femto- and Attosecond Science
International Day of Women and Girls in Science- SSPh asked female scientists about their experiences
New scientific highlight- by MUST PIs Milne, Standfuss and Schertler
EU XFEL Young Scientist Award for Camila Bacellar,beamline scientist and group leader of the Alvra endstation at SwissFEL
Prizes for Giulia Mancini and Rebeca Gomez CastilloICO/IUPAP Young Scientist Prize in Optics & Ernst Haber 2021
Nobel Prize in Chemistry awarded to RESOLV Member Benjamin List- for the development of asymmetric organocatalysis
NCCR MUST at Scientifica 2021- Lightning, organic solar cells, and virtual molecules
#NCCRWomen- NCCR MUST celebrates 50 years women’s right to vote in Switzerland

Structural and allosteric transitions of heme protein sensors probed from picosecond to second

Date Di, 19.11.2019 - Di, 19.11.2019
Time 14:00h
Speaker Dr. Michel Négrerie, Ecole Polytechnique, France
Location EPF Lausanne Campus, CH G1 495 2201
Program The human receptor of nitric oxide (NO), named soluble guanylate cyclase (sGC), is a heme protein present in numerous cells and possesses two functional remote domains: the sensing domain which harbours the heme cofactor and the catalytic domain which synthesizes cGMP from GTP. The synthesis of the cellular messenger cGMP in the catalytic domain is triggered upon NO binding to the heme thanks to a structural allosteric change. Structural transitions are induced at the heme level by NO binding to and release from sGC. I will present how time-resolved absorption spectroscopy in the UV-visible range in the broad time range from 1 picosecond to 1 second allowed to probe localized structural events. Structural allosteric transitions of the NO-receptor, correlated with NO dynamics, were also detected. Time-resolved absorption spectroscopy was also used to decipher the mechanism of action of sGC activators which are artificial drugs and act in the absence of NO. The dynamics of heme-bound side-chains, like methionine as the 6th iron ligand in mitochondrial cytochrome c, could be measured and revealed a conformational change in the protein.
Download (75 KB)
(75 KB)
Link LACUS Seminars
NCCR MUST Office : ETHZ IQE/ULP-HPT H3 | Auguste-Piccard-Hof 1 | 8093 Zurich | E-Mail
The National Centres of Competence in Research (NCCR) are a research instrument of the Swiss National Science Foundation