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Lipid membrane bioactivity – novel insights from optical super-resolution (STED-FCS) microscopy

Date Do, 07.05.2015 - Do, 07.05.2015
Time 16.30
Speaker Prof. Christian Eggeling, University of Oxford, United Kingdom
Location EPF Lausanne, CH G1 495
Program Molecular interactions in the plasma membrane of living cells are key in cellular signalling. Especially, lipids or diffusional restrictions by the cortical cytoskeleton are considered to play a functional part in a whole range of membrane-associated processes. The direct and non-invasive observation of such interactions in living cells is often impeded by principle limitations of conventional far-field optical microscopes, specifically with respect to limited spatio-temporal resolution. Here, I show how novel details of molecular membrane dynamics can be obtained by using advanced microscopy approaches such as the combination of super-resolution STED microscopy with fluorescence correlation spectroscopy (STED-FCS). These include new insights into the lipid “raft” theory, and on the role of plasma membrane and cytoskeleton organization in the triggering of immune cells, specifically during T-cell activation.
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Link EPFL Conferences
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